Coding

Part:BBa_J62005:Design

Designed by: John Dueber   Group: Keasling Lab   (2008-07-26)

{HMGS} HMG CoA Synthase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

cloned from a template in the Keasling lab with the following primers: JD_F48. SYN BB F. ggctaaGAATTCatgAGATCTATGAAACTGAGCACCAAGCTG JD_F49. SYN BB R. taacgtCTCGAGtcaGGATCCCTTTTTGACGTCATAAGAACG

References